Publication Abstracts

Kiang et al. 2022

Kiang, N.Y., W.D. Swingley, D. Gautam, J.T. Broddrick, D. Repeta, J.F. Stolz, R.E. Blankenship, B.M. Wolf, A.M. Detweiler, K.A. Miller, J.J. Schladweiler, R. Lindeman, and M.N. Parenteau, 2022: Discovery of chlorophyll d: Isolation and characterization of a far-red cyanobacterium from the original site of Manning & Strain (1943) at Moss Beach, California. Microorganisms, 10, no. 4, 819, doi:10.3390/microorganisms10040819.

We have isolated a chlorophyll d-containing cyanobacterium from the intertidal field site at Moss Beach, on the coast of Central California, USA, where Manning & Strain (1943) originally discovered this far-red chlorophyll. Here we present the cyanobacterium's environmental description, culturing procedure, pigment composition, ultrastructure, and full genome sequence. Among cultures of far-red cyanobacteria obtained from red algae from the same site, this strain was an epiphyte on a brown macroalgae. Its Qy in vivo absorbance peak is centered at 704-705 nm, the shortest wavelength thus far observed among the various Acaryochloris strains known. Its Chl a/Chl d ratio was 0.01, with Chl d accounting for 99% of the total Chl d and Chl a mass. TEM imagery indicates the absence of phycobilisomes, corroborated by both pigment spectra and genome analysis. The Moss Beach strain codes for only a single set of genes for producing allophycocyanin. Genomic sequencing yielded a 7.25 Mbp circular chromosome and 10 circular plasmids ranging from 16 kbp to 394 kbp. We have determined that this strain shares high similarity with strain S15, an epiphyte of red algae, while its distinct gene complement and ecological niche suggest that this strain could be the closest known relative to the original Chl d source of Manning and Strain. The Moss Beach strain is designated Acaryochloris sp. (marina) strain Moss Beach.

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BibTeX Citation

@article{ki01300f,
  author={Kiang, N. Y. and Swingley, W. D. and Gautam, D. and Broddrick, J. T. and Repeta, D. and Stolz, J. F. and Blankenship, R. E. and Wolf, B. M. and Detweiler, A. M. and Miller, K. A. and Schladweiler, J. J. and Lindeman, R. and Parenteau, M. N.},
  title={Discovery of chlorophyll d: Isolation and characterization of a far-red cyanobacterium from the original site of Manning & Strain (1943) at Moss Beach, California},
  year={2022},
  journal={Microorganisms},
  volume={10},
  number={4},
  pages={819},
  doi={10.3390/microorganisms10040819},
}

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RIS Citation

TY  - JOUR
ID  - ki01300f
AU  - Kiang, N. Y.
AU  - Swingley, W. D.
AU  - Gautam, D.
AU  - Broddrick, J. T.
AU  - Repeta, D.
AU  - Stolz, J. F.
AU  - Blankenship, R. E.
AU  - Wolf, B. M.
AU  - Detweiler, A. M.
AU  - Miller, K. A.
AU  - Schladweiler, J. J.
AU  - Lindeman, R.
AU  - Parenteau, M. N.
PY  - 2022
TI  - Discovery of chlorophyll d: Isolation and characterization of a far-red cyanobacterium from the original site of Manning & Strain (1943) at Moss Beach, California
JA  - Microorganisms
VL  - 10
IS  - 4
SP  - 819
DO  - 10.3390/microorganisms10040819
ER  -

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